rabbit polyclonal anti erk  (Cell Signaling Technology Inc)

Journal: International Dental Journal

Article Title: 4632427E13Rik Facilitates Jaw Marrow-Derived Mesenchymal Stem Cells Osteogenesis and Angiogenesis Under Hypoxia Through miR-34a-5p/Aldoa/Hif-1α Pathway

doi: 10.1016/j.identj.2025.109364

Figure Lengend Snippet: Aldoa promotes osteogenesis and angiogenesis via the ERK/Hif-1α pathway. A, Alizarin red staining (14 days) and ALP staining (7 days). B, The gene expression levels of Runx2, Alp and Ocn were determined by qRT-PCR. β-actin was used as an internal reference gene. C, Tube formation assay was performed in the presence of CM. The scale bars represent 100 μm. D, The mRNA expression levels of Vegf and CD31 were analysed by quantitative RT-PCR. β-actin was used as an internal reference gene. E, Immunofluorescence staining was performed to detect Vegf expression after being treated by CM. The scale bars represent 25 μm. F, A Kyoto Encyclopaedia of Genes and Genomes (KEGG) enrichment analysis was performed to determine the top related pathways involving these differentially expressed mRNAs. G and H, The protein expression levels of Aldoa, Hif-1α and the phosphorylated of ERK, JUK and p38 among hypoxia, normoxia and hypoxia + si-Aldoa groups were determined by western blot. Semiquantitative analysis of the p-ERK/ERK, p-JUK/JUK and p38/p-p38 ratios were shown. β-actin was used as an internal reference gene. All data were expressed as means ± SD. * P < .05, ** P < .01.

Article Snippet: Rabbit anti-Aldoa (1:1000, 11217-1-AP), rabbit anti-Hif-1α (1:1000, ab179483), rabbit anti-Runx2 (1:1000, 8486S), rabbit anti-CD31 (1:1000, 77699S), rabbit anti-Ocn (1:1000, bs-4917R), rabbit anti-Alp (1:1000, bs-1535R), rabbit anti-Vegf (1:1000, bs-1313R), rabbit anti-ERK (1:1000, bsm-33337M), rabbit anti-p-ERK (1:1000, bs-1646R), rabbit anti-p38 (1:1000, bs-0637R), rabbit anti-p-p38 (1:1000, bs-0636R), rabbit anti-JNK (1:1000, bs-2592R), rabbit anti-p-JNK (1:1000, bs-1640R), and mouse anti-β-actin (1:2000, AF7018) were purchased from Proteintech, Abcam, Cell Signalling Technology, and Bioss, respectively.

Techniques: Staining, Gene Expression, Quantitative RT-PCR, Tube Formation Assay, Expressing, Immunofluorescence, Western Blot

Journal: Frontiers in Cellular Neuroscience

Article Title: Differential microglial dynamics and neuroinflammation underlying neuropathic pain in the central nervous system: comparative insights from spinal cord injury and compressive myelopathy models

doi: 10.3389/fncel.2026.1769004

Figure Lengend Snippet: Expression of pain-related molecules in the spinal cord. (A) In the SCI model, p-p38 and p-ERK1/2 expression colocalized with CD11b at the lesion site peaked at 2 weeks post-injury and increased in the lumbar enlargement during the chronic phase (12 weeks). Scale bars = 50 μm. (B) In the DCM model, expression of p-p38 and p-ERK1/2 colocalized with CD11b progressively increased at the compression site, with little change in the lumbar enlargement. Scale bars = 50 μm.

Article Snippet: The primary antibodies (Abs) used were rabbit anti-phospho-p38 MAP kinase (p-p38) polyclonal Ab (1:400, Cell Signaling Technology, Beverly, MA); rabbit anti- p44/42 MAPK (p-ERK1/2) polyclonal Ab (Cell Signaling Technology); and rabbit anti-CD11b Ab (1:50, Abcam, Cambridge, UK).

Techniques: Expressing

Journal: Frontiers in Cellular Neuroscience

Article Title: Differential microglial dynamics and neuroinflammation underlying neuropathic pain in the central nervous system: comparative insights from spinal cord injury and compressive myelopathy models

doi: 10.3389/fncel.2026.1769004

Figure Lengend Snippet: Expression of pain-related molecules in the brain. (A) In the SCI model, there was significant expression of p-p38 and p-ERK1/2 colocalized with CD11b in the hippocampus and amygdala during the chronic phase (12 weeks) and in the thalamus at both acute and chronic time points. Scale bars = 50 μm. (B) In the DCM model, expression of pain-related molecules increased in the hippocampus, amygdala, and thalamus under moderate compression (18 weeks). Scale bars = 50 μm.

Article Snippet: The primary antibodies (Abs) used were rabbit anti-phospho-p38 MAP kinase (p-p38) polyclonal Ab (1:400, Cell Signaling Technology, Beverly, MA); rabbit anti- p44/42 MAPK (p-ERK1/2) polyclonal Ab (Cell Signaling Technology); and rabbit anti-CD11b Ab (1:50, Abcam, Cambridge, UK).

Techniques: Expressing